1997 - Ph.D. Biological Science, Hiroshima University, Japan
1994 - M.S. Plant Pathology, Tokyo University of Agriculture, Japan
Research Interest - Cell Migration
Cell migration plays a key role in both the physiological and the pathophysiological function of the cells including development, wound healing, immunity, cancer metastasis and cardiovascular disease. In the developed world, cancer metastasis and cardiovascular disease remain the two leading causes of death.
- Regulation of ZIP Kinase on Cell Motility and Contractility
- Regulation of Nonmuscle myosin II isoforms during cell migration in 3D environments
A lack of our knowledge about the mechanisms of cell migration in three-dimensional (3D) environments leads to limited developing therapeutic approaches for treating cancer metastasis and cardiovascular disease. In fact, several previous studies demonstrated that cells plated in 3D matrices have dramatically different actin cytoskeletal structure and focal adhesion compositions from conventional two-dimensional (2D) cultured cells. Therefore, the information provide from the 3D cell migration study will contribute to understanding of disease states such as cancer metastasis, fibrosis and cardiovascular disease as well as normal physiological processes such as development, wound healing, and immunity.
A number of studies on the complex behavior of cell migration have been conducted to investigate how cytoskeletal structures are regulated during this process. Although cytoskeletal structures dictating cell shape and motile behavior have been studied extensively in 2D cell migration systems, the cellular architectures of motile cells in 3D environments are quite different from those in 2D environments and to date have not been thoroughly investigated. Cells under physiological environment interact with extracellular matrices on all surfaces, not just on the basal surface. This raises a question that there may be a distinct mechanism in migrating cells between flat cells on 2D surfaces and more rounded spindle-shaped cells in 3D matrices. To address this issue, we propose to study 3D cell migration by using a multidisciplinary approach involving molecular techniques, 3D migration assay, time-lapse confocal microscopy and image analysis.
Komatsu, S., Yano, T., Shibata, M., Tuft, R.A., and Ikebe, M. Effects of the regulatory light chain phosphorylation of myosin II on mitosis and cytokinesis of mammalian cells. J. Biol. Chem. 275, 34512-34520 (2000).
Ikebe, M., Komatsu, S., Woodhead, J.L., Mabuchi, K., Ikebe, R., Saito, J., Craig, R., and Higashihara, M. The tip of the coiled-coil rod determines the filament formation of smooth muscle and non-muscle myosin. J. Biol. Chem. 276, 30293-30300 (2001).
Komatsu, S., and Ikebe, M. ZIP Kinase Is Responsible for the Phosphorylation of Myosin II and Necessary for Cell Motility in Mammalian Fibroblasts. J. Cell Biol. 165, 243-254 (2004). (This paper has been citied in “Faculty of 1000”)
Komatsu, S.,* and Ikebe, M.* The Phosphorylation of Myosin II at the Ser1 and Ser2 Is Critical for Normal Platelet-derived Growth Factor induced Reorganization of Myosin Filaments. Mol. Biol. Cell 18, 5081-5090 (2007). (*corresponding author)
Jung, H.S., Komatsu, S., Ikebe, M. and Craig, R. Head–Head and Head–Tail Interaction: A General Mechanism for Switching Off Myosin II Activity in Cells. Mol. Biol. Cell 19, 3234-3242 (2008).
Komatsu, S.,* and Ikebe, M. ZIPK is critical for the motility and contractility of VSMC through the regulation of Nonmuscle Myosin II isoforms. Am. J. Physiol. Heart Circ. Physiol. 306, H1275-1286 (2014). (*corresponding author)